Utilization of Alternative Buffer Solutions for Staining Thin Blood smears by the Giemsa, Wright stain and Romanowsky method

To make a reliable diagnosis on haematological examination, it is necessary to examine further the morphology of blood cells previously stained in procedure for staining thin smears using buffer solution with standard pH 6.4 6.8. One problems that may occur laboratory reagents are damaged, past expiration date or running out, so alternative buffers needed cheap, fast and easy obtain. This study aims evaluate results from buffers. Study used quasi-experimental method, bottled mineral water following SNI-01-0553 2006. The colour produced by some these almost equivalent phosphate buffer. percentage assessment codes B, C, D, E F compared control (A) were 62.67%, 92.00%, 82.67%, 80.00% 88, 00%, 68.00%. use Giemsa, Wright stain, Romanowsky method can be done as an sample F, while B G cannot used. applied personnel urgent situations limited equipment material facilities.